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CABRI:DSMZ_MUTZ |
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Accession_number |
ACC 28
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Cell_line_name |
4H1-A7
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Brief_description |
confirmed as mouse with IEF of MDH, NP, PEP B established by fusion of SP2/0-
AG14 myeloma cells with BALB/c mouse spleen cells immunized with purified canine
adenovirus type 2 (CAV-2, Manhattan strain); the secreted antibody (IgG 2a ) binds to
and neutralizes CAV-2 and is type-specific
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Description |
mouse hybridoma (anti-canine adenovirus 2) established by fusion of SP2/0-AG14
myeloma cells with BALB/c mouse spleen cells immunized with purified canine
adenovirus type 2 (CAV-2, Manhattan strain); the secreted antibody (IgG 2a ) binds to
and neutralizes CAV-2 and is type-specific confirmed as mouse with IEF of MDH, NP,
PEP B
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Depositor |
Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
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Morphology |
single cells in suspension
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Culture_conditions |
92.5% Dulbecco's MEM (4.5 g/L glucose) + 5% horse serum + 2.5% FBS maintain
at 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:2 every 2-3 days at 37C with 5%
CO2 maximum density at 1.0-1.5 x 106 cells/ml
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Viruses |
ELISA: reverse transcriptase negative; PCR: SMRV -
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Properties |
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Release_conditions |
-
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Hazard |
-
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Storage |
frozen with 75% medium, 10% horse serum, 5% FBS, 10% DMSO at about 5 x 106
cells/ampoule
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Mycolasma |
negative in DAPI, microbiological culture, RNA hybridization assays
Cytogenetics murine hypertetraploid/pentaploid karyotype with 5% polyploidy - 91-98<4n> - 3
centric fusion markers and 1-3 dmin present in most cells
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Bibliographic_reference |
Whetstone et al., Intervirology, 23: 31 (1985) [PMID: 3884549
] |
Whetstone et al., Vet Microbiol, 16: 31 (1988) [PMID: 3281371]
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